Quantitative assessment of microvasculopathy in arcAβ mice with USPIO-enhanced gradient echo MRI


Journal article


J. Klohs, A. Deistung, G. D. Ielacqua, A. Seuwen, D. Kindler, F. Schweser, M. Vaas, A. Kipar, J. Reichenbach, M. Rudin
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 2015

Semantic Scholar DOI PubMedCentral PubMed
Cite

Cite

APA
Klohs, J., Deistung, A., Ielacqua, G. D., Seuwen, A., Kindler, D., Schweser, F., … Rudin, M. (2015). Quantitative assessment of microvasculopathy in arcAβ mice with USPIO-enhanced gradient echo MRI. Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism.

Chicago/Turabian
Klohs, J., A. Deistung, G. D. Ielacqua, A. Seuwen, D. Kindler, F. Schweser, M. Vaas, A. Kipar, J. Reichenbach, and M. Rudin. “Quantitative Assessment of Microvasculopathy in ArcAβ Mice with USPIO-Enhanced Gradient Echo MRI.” Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (2015).

MLA
Klohs, J., et al. “Quantitative Assessment of Microvasculopathy in ArcAβ Mice with USPIO-Enhanced Gradient Echo MRI.” Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism, 2015.


Abstract

Magnetic resonance imaging employing administration of iron oxide-based contrast agents is widely used to visualize cellular and molecular processes in vivo. In this study, we investigated the ability of R 2 * and quantitative susceptibility mapping to quantitatively assess the accumulation of ultrasmall superparamagnetic iron oxide (USPIO) particles in the arcAβ mouse model of cerebral amyloidosis. Gradient-echo data of mouse brains were acquired at 9.4 T after injection of USPIO. Focal areas with increased magnetic susceptibility and R 2 * values were discernible across several brain regions in 12-month-old arcAβ compared to 6-month-old arcAβ mice and to non-transgenic littermates, indicating accumulation of particles after USPIO injection. This was concomitant with higher R 2 * and increased magnetic susceptibility differences relative to cerebrospinal fluid measured in USPIO-injected compared to non-USPIO-injected 12-month-old arcAβ mice. No differences in R 2 * and magnetic susceptibility were detected in USPIO-injected compared to non-injected 12-month-old non-transgenic littermates. Histological analysis confirmed focal uptake of USPIO particles in perivascular macrophages adjacent to small caliber cerebral vessels with radii of 2–8 µm that showed no cerebral amyloid angiopathy. USPIO-enhanced R 2 * and quantitative susceptibility mapping constitute quantitative tools to monitor such functional microvasculopathies.